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By John A. Borrelli, Ylenia D. Giannini

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O. A. Chiocca, Improved HSV-1 amplicon packaging system using ICP27-deleted, oversized HSV-1 BAC DNA. Methods Mol Med, 2003. 76: p. 51-60. 40 Matias E. Melendez, Alejandra I. Aguirre, Maria V. Baez et al. , Improved helper virus-free packaging system for HSV amplicon vectors using an ICP27-deleted, oversized HSV-1 DNA in a bacterial artificial chromosome. Mol Ther, 2001. 3(4): p. 591-601. , A new method to propagate defective HSV-1 vectors. Nucleic Acids Res, 1988. 16(12): p. 5690. , An efficient deletion mutant packaging system for defective herpes simplex virus vectors: potential applications to human gene therapy and neuronal physiology.

Improved helper virus-free packaging system for HSV amplicon vectors using an ICP27-deleted, oversized HSV-1 DNA in a bacterial artificial chromosome. Mol Ther, 2001. 3(4): p. 591-601. , A new method to propagate defective HSV-1 vectors. Nucleic Acids Res, 1988. 16(12): p. 5690. , An efficient deletion mutant packaging system for defective herpes simplex virus vectors: potential applications to human gene therapy and neuronal physiology. Proc Natl Acad Sci U S A, 1990. 87(22): p. 8950-4. , Generation of high-titer defective HSV-1 vectors using an IE 2 deletion mutant and quantitative study of expression in cultured cortical cells.

In both preparations from Mapt(–/–) mice infected with the vector bearing the MAPT transgene, the tau protein was expressed as detected by ELISA and immunocytochemistry, and the sensitivity of Mapt(–/–) neurons to Aβ peptide was restored [113]. As stated by the 30 Matias E. Melendez, Alejandra I. Aguirre, Maria V. Baez et al. authors, the faithful retention of gene expression and phenotypic complementation by this system provides a novel and powerful approach to analyze neurological disease genes.

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