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By Gaylon S. Campbell

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"well written and illustrated"   -- Bulletin of the yankee Meteorological Society

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The Preparation of Pi 1. 9, for 1 h at 37  C. 2. Phenol/chloroform extract the reaction once. The aqueous layer contains radioactive inorganic Pi. ACKNOWLEDGMENT Support for this work was provided by NIH grant GM67005 to M. K. REFERENCES Anderson, J. S. , and Parker, R. P. (1998). The 30 to 50 -degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 30 to 50 -exonucleases of the exosome complex. EMBO J. 17, 1497–1506. Bessman, M. , Frick, D.

LEE AND REIKO T. LEE VOLUME 364. Nuclear Receptors Edited by DAVID W. RUSSELL AND DAVID J. MANGELSDORF VOLUME 365. Differentiation of Embryonic Stem Cells Edited by PAUL M. WASSAUMAN AND GORDON M. KELLER VOLUME 366. Protein Phosphatases Edited by SUSANNE KLUMPP AND JOSEF KRIEGLSTEIN VOLUME 367. Liposomes (Part A) Edited by NEJAT DU¨ZGU¨NES, VOLUME 368. Macromolecular Crystallography (Part C) Edited by CHARLES W. , AND ROBERT M. SWEET VOLUME 369. Combinational Chemistry (Part B) Edited by GUILLERMO A.

2004). Cytoplasmic foci are sites of mRNA decay in human cells. J. Cell Biol. 165, 31–40. Decker, C. , and Parker, R. (1993). A turnover pathway for both stable and unstable mRNAs in yeast: Evidence for a requirement for deadenylation. Genes Dev. 7, 1632–1643. 20 Shin-Wu Liu et al. Decker, C. , and Parker, R. (1994). Mechanisms of mRNA degradation in eukaryotes. Trends Biochem. Sci. 19, 336–340. , and Parker, R. (1999). The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif.

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