By Donna H. Wang
Michigan nation Univ., East Lansing. interprets wisdom into reproducible tools than let biomedical researchers to discover the function of angiotensin in overall healthiness and affliction. Covers genetic manipulation of exact genes. comprises tools for sensible reviews of the renin-angiotensin procedure. DNLM: Renin - Angiotensin System-physiology
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Agtr1a+/– ES cells 2. Hygromycin resistant feeder cells; NHL-7 (kind gift from Dr. H. Kondo, Osaka University, Osaka, Japan; ref. 19) 3. An Agtr1a targeting vector containing the hygromycin resistant gene (Hgr). 4. ) 5. ) 6. , Palo Alto, CA) 7. ES cell medium [Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 17% fetal calf serum (Gibco-BRL Products)] 8. The system for microinjection a. Micromanipulators b. Inverted fixed-stage microscope c. Cooling stage and injection chamber for microscope 9.