By Brigitte Schultze
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Extra resources for Autoradiography At the Cellular Level. Physical Techniques in Biological Research
5% T C A ) fixed and paraffin-embedded sections of liver and brain after application of r e labeled amino acids to the isolation procedure of nucleic acids according to Schneider's method (1945) and found an activity loss of only 0% to 3%, measured with the Geiger counter. Ostrowski et al. (1961) found a 1% loss of protein mass in liver and kidney after fixation with 4 % for malin, and Merriam (1958) reported 6-12% weight loss in liver, muscle, and brain with the same fixative. In order to eliminate the labeled free amino acid from the tissue as completely as possible it is advisable to add the corresponding nonradio active amino acid in excess to the fixative and all solutions subsequently used.
For thick emulsion layers 20-30 min of development may be required, depending on the temperature. In some cases it might be necessary to soak the thick emulsion in distilled water prior to development to make it penetrable to the developer. Yagoda (1955) described a special isothermal processing of thick nuclear emulsion that provides uniform development throughout the entire thickness. A similar procedure was recommended by Guidotti and Setti (1956). This temperature-development method facilitates the work with electron tracks.
1964). A new method for better recognition and photographic reproduction of human chromosomes in tritium autoradiograms by removing the silver and/or digesting the gelatin of the film was described by Bianchi et al. (1964) and Froland (1965). VI. Autoradiography of Water-Soluble Substances A. PRECIPITATION M E T H O D In order to prevent the leaching of radioactive water-soluble substances, 32 several methods have been used in autoradiography. , 1949). Br was precipitated by alco holic silver nitrate (Winteringham et al, 1950).