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Which one of the following binds to specific nucleotide sequences that are upstream of the start site of transcription? a. b. c. d. e. RNA polymerase Primase Helicase Histone protein Restriction endonuclease 51. Template-directed RNA synthesis occurs in which of the following? a. b. c. d. e. Point mutation Triplet repeat expansion Initiation of the polymerase chain reaction Expression of oncogenes Repair of thymine dimers 52. Which of the following most correctly describes mammalian messenger RNAs?

A. b. c. d. e. Formylated methionyl-tRNA is important for initiation of translation Single mRNAs specify more than one gene product Cycloheximide blocks elongation during translation Cytosolic ribosomes are smaller than those found in prokaryotes Erythromycin inhibits elongation during translation 57. Modification of mRNA so that a signal sequence is added to the amino terminus of the cytosolic protein, α-globin, results in a. b. c. d. e. qxd 44 11/14/01 10:37 AM Page 44 Biochemistry and Genetics 58.

They are found both free in the cytoplasm and bound to membranes c. They are bound together so tightly they cannot dissociate under physiologic conditions d. They are composed of RNA, DNA, and protein e. They are composed of three subunits of unequal size 62. Guanosine triphosphate (GTP) is required by which of the following steps in protein synthesis? a. b. c. d. e. qxd 11/14/01 10:37 AM Page 45 Gene Expression 45 63. Erythromycin is the antibiotic of choice when treating respiratory tract infections in legionnaire’s disease, whooping cough, and Mycoplasma-based pneumonia because of its ability to inhibit protein synthesis in certain bacteria by a.

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