By Daniel F. Bogenhagen (auth.), Jeffrey A. Stuart (eds.)
Since the ebook of the 1st version, the variety of targeted heritable mtDNA mutations well-known as being linked to bioenergetic disorder, mobile dying and sickness has grown. Likewise, our realizing of the elemental biology of somatic mtDNA mutations keeps to enhance. In Mitochondrial DNA: tools and Protocols, moment Edition, experts from 8 international locations proportion their services, delivering designated protocols for learning many elements of mtDNA. the quantity is split into 3 sections, which hide the transduction of knowledge from mtDNA to functionally lively breathing complexes, mitochondrial reactive oxygen species (ROS) creation in addition to mtDNA harm and its fix, and the identity and quantification of heteroplasmic mtDNA mutations. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, easily reproducible laboratory protocols, and notes sections, highlighting tips about troubleshooting and fending off identified pitfalls.
Comprehensive and authoritative, Mitrochondrial DNA: equipment and Protocols, moment Edition can provide to assist researchers in additional increasing our wisdom of this important zone of mobile biology.