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By John G. Day, Mark R. McLellan

This booklet offers distinctive protocols for all of the most recent methodologies used to guarantee the long term biostorage of a various variety of organic fabrics.

constructed in professional laboratories, the protocols were painstakingly perfected through the years to supply time-tested, step by step directions that be certain powerful and reproducible effects. each one protocol offers with the maintenance of an organelle, mobilephone, or tissue style, and is offered even to the nonspecialist as a result of its cookbook process. Novel protocols could be effortlessly built with the aid of the "hands-on" Notes sections.

Cryopreservation and Freeze-Drying Protocols is an vital reference paintings for either the person researcher, and all those that are looking to determine or increase biostorage structures of their laboratories. Its functions variety from microbial tradition collections, botanic gardens, and zoos to animal husbandry, aquaculture, drugs, human fertilization, and telephone and molecular biology.

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Work with mutant strains of S. pombe at the NCYC has also shown that genetic stability is also very good. If the racking system used holds many cryotubes they will all be exposed to higher temperatures when the racking system is removed from the liquid nitrogen to recover a straw. Care should be taken to minimize this time as much as possible. Work done at the NCYC has suggested that using straws sealed mside cryotubes offers considerable protection against short Cryopreservation of Yeast Cultures 47 exposure to higher temperatures.

K. Appl. Environ. Microbial. 35,84-88. 5. Pearson, B. , Jackman, P. J. , Painting, K. , and Morris, G J. (1990) Stability of genetically manipulated yeasts under different cryopreservation regimes. Ctyo-Lett. 11,205-210. 6. Diller, R. R. and Knox, J. M. (1983) Automated computer-analysis of cell-size changes during cryomicroscope freezing-a biased trident convolution mask technique. Cryo-Lett. 4,77-92. 7. Morris, G. , Coulson G. , and Clarke K. J. (1988) Freezing injury in Saccharomyces cerevisiae-the effect of growth conditions.

Totowa, NJ 31 32 Kawamura et al. 2. Materials 1. Growth medium: YM agar medium (see Notes 1 and 2). Dissolve 3 g yeast extract, 3 g malt extract, 5 g peptone, 10 g glucose, and 20 g agar in 1 L of distilled water (pH not adjusted). Pour 7 mL of YM agar medium (see Note 3) into a test tube, plug with cotton wool, autoclave at 120°C for 15 min, then cool to form an agar slant. 2. Ampules: Tubular glass ampule (see Notes 4-6) (8-mm outer diameter, 15cm long) (see Fig. 1). After washing in detergent, rinse in distilled water and dry.

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