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Expression of the Gli genes in the normal and transgenic rat brain. (A) Coronal sections of E12, WT rat brain hybridized with probes to Gli-1, -2 and -3. D marks dorsal mid-hindbrain and V marks ventral mid-hindbrain. (B) Schematic drawing illustrating the regions of normal (WT) and ectopic dorsal (TG) expression in the brain (solid lines in embryos). Arrowheads mark region of overgrowth in the TG embryos, and the comparable region in the WT embryos. 108,109 Finally, ectopic expression of human Gli-1 in the dorsal mid-hindbrain region of the neural tube in transgenic mice was shown to result in profound morphological changes due to overgrowth of the mid-hindbrain region of the brain107(Fig.

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