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By Antonio Alonso

This quantity within the tools in Molecular Biology sequence covers tools in forensic DNA profiling, together with protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable areas HV1 and HV2.

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Butler JM, Shen Y, McCord BR. The development of reduced size STR amplicons as tools for analysis of degraded DNA. J Forensic Sci 2003;48(5):1054–64. 4. Wenk RE, Chiafari FA, Gorlin J, Polesky HF. Better tools are needed for parentage and kinship studies. Transfusion 2003;43:979–81. 5. Wenk RE, Gjertson DW, Chiafari FA, Houtz T. The specific power of parentage exclusion in a child’s blood relatives. Transfusion 2005;45:440–4. 6. Goodwin W, Ballard D, Simpson K, Thacker C, Syndercombe Court D, Gow J.

POP™-4 or POP™-6 Polymer (Applied Biosystems). 7. 16-capillary ABI Prism® 3130xl Genetic Analyzer (Applied Biosystems). 4. Data Analysis Software 1. 2 (Applied Biosystems) (see Note 6). 2. GeneMapper bins and panels (available on STRBase) (see Note 7). R. Hill 3. 1. Primer Set Preparation 1. Fluorescently labeled forward and unlabeled reverse primers are delivered lyophilized and must be reconstituted with TE−4 buffer (see Note 8) to the following concentrations: Unlabeled reverse primers = 200 μM.

I would also like to express appreciation to the Applied Genetic group members for technical assistance with the 26plex assay, in particular Margaret Kline for the DNA Sequencing work and Dave Duewer for data analysis support. I would also like to acknowledge the National Institute of Justice for their funding to our NIST Group through the NIST Law Enforcement Standards Office interagency agreement 2008DN-R-121. The opinions and assertions contained herein are solely those of the author and are not to be construed as official or as views of the US Department of Commerce.

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