By Janos Minarovits, Hans Helmut Niller
This quantity explores facts from the functions of molecular organic tools and the functions of contemporary immunological and cytogenetic tools in Epstein-Barr Virus (EBV) that might provide readers attainable new ideas to the unresolved difficulties within the EBV box. Chapters during this publication conceal themes akin to: viral existence cycle, latency, EBV-associated ailments and EBV diagnostics; in vitro equipment together with organotypic cultures for the research of EBV-epithelial mobilephone interactions; id of the interacting viral and mobile proteins utilizing affinity purification-mass spectrometry equipment; 3D telomere FISH; transcription research utilizing high-throughput RNA sequencing, qPCR and nuclear run-on assay; research of viral and mobile microRNAs; isolation and characterization of exosomes and the evaluate in their functionality; characterization of the viral genome by means of terminal repeat research and sequencing; using chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the research of Zta-DNA interactions; epigenetic research by means of bisulfite sequencing and ChIP; novel in vivo types for the examine of EBV an infection; and the way immunological, virological, tissue tradition and molecular tools will be mixed to yield sturdy production Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative illnesses (PTLD). Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and pointers on troubleshooting and keeping off identified pitfalls.
Cutting-edge and accomplished, Epstein Barr-Virus: equipment and Protocols is a important source for someone who's attracted to this attention-grabbing and evolving field.
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83. 84. 85. cells and their relationship to human B-cell lymphomas. Blood 120(11):2240–2248. 1182/blood-2012-03-415380 Schmitz R, Ceribelli M, Pittaluga S, Wright G, Staudt LM (2014) Oncogenic mechanisms in Burkitt lymphoma. Cold Spring Harb Perspect Med 4(2):a014282. a014282 Robbiani DF, Deroubaix S, Feldhahn N, Oliveira TY, Callen E, Wang Q, Jankovic M, Silva IT, Rommel PC, Bosque D, Eisenreich T, Nussenzweig A, Nussenzweig MC (2015) Plasmodium infection promotes genomic instability and AID-dependent B cell lymphoma.
Nature 310(5974):207–211 5. Lin Z, Wang X, Strong MJ, Concha M, Baddoo M, Xu G, Baribault C, Fewell C, Hulme W, Hedges D, Taylor CM, Flemington EK (2013) Whole-genome sequencing of the Akata and Mutu Epstein-Barr virus strains. J Virol 87(2):1172–1182. 02517-12 6. Kwok H, Wu CW, Palser AL, Kellam P, Sham PC, Kwong DL, Chiang AK (2014) Genomic diversity of Epstein-Barr virus genomes isolated from primary nasopharyngeal carcinoma biopsy samples. J Virol 88(18):10662–10672. 1128/JVI. 01665-14 7. Palser AL, Grayson NE, White RE, Corton C, Correia S, Ba Abdullah MM, Watson SJ, Cotten M, Arrand JR, Murray PG, Allday MJ, Rickinson AB, Young LS, Farrell PJ, Kellam P (2015) Genome diversity of Epstein-Barr virus from multiple tumor types and normal infection.
4459 10. Tsao SW, Tsang CM, To KF, Lo KW (2015) The role of Epstein-Barr virus in epithelial malignancies. J Pathol 235(2):323–333. 4448 11. Niller HH, Banati F, Salamon D, Minarovits J (2016) Epigenetic alterations in EpsteinBarr virus-associated diseases. Adv Exp Med Biol 879:39–69. 1007/978-3-31924738-0_3 12. Longnecker R, Kieff E, Cohen JI (2013) Epstein Barr virus. In: Knipe DM, Howley PM (eds) Fields virology, 6th edn. Lippincott Williams & Wilkins, Philadelphia, pp 1898–1959 13. Thorley-Lawson DA (2001) Epstein-Barr virus: exploiting the immune system.