Download Epstein Barr Virus: Methods and Protocols by Janos Minarovits, Hans Helmut Niller PDF

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By Janos Minarovits, Hans Helmut Niller

This quantity explores facts from the functions of molecular organic tools and the functions of contemporary immunological and cytogenetic tools in Epstein-Barr Virus (EBV) that might provide readers attainable new ideas to the unresolved difficulties within the EBV box. Chapters during this publication conceal themes akin to: viral existence cycle, latency, EBV-associated ailments and EBV diagnostics; in vitro equipment together with organotypic cultures for the research of EBV-epithelial mobilephone interactions; id of the interacting viral and mobile proteins utilizing affinity purification-mass spectrometry equipment; 3D telomere FISH; transcription research utilizing high-throughput RNA sequencing, qPCR and nuclear run-on assay; research of viral and mobile microRNAs; isolation and characterization of exosomes and the evaluate in their functionality; characterization of the viral genome by means of terminal repeat research and sequencing; using chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the research of Zta-DNA interactions; epigenetic research by means of bisulfite sequencing and ChIP; novel in vivo types for the examine of EBV an infection; and the way immunological, virological, tissue tradition and molecular tools will be mixed to yield sturdy production Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative illnesses (PTLD). Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and pointers on troubleshooting and keeping off identified pitfalls.

Cutting-edge and accomplished, Epstein Barr-Virus: equipment and Protocols is a important source for someone who's attracted to this attention-grabbing and evolving field.

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