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By Michele Lees

Textual content studies present and rising concepts in nutrition authenticity and traceability. For execs operating in meals protection and caliber.

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SEITZ O (2000), `Solid-phase synthesis of doubly labelled peptide nucleic acids as probes for the real-time detection of hybridisation', Angew Chemistry International Editors, 39, 3249±3252. SINGH S K, NIELSEN P, KOSHKIN A, WENGEL J (1998), `LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition', Chemistry Communications, 455±456. È THY J, HU È BNER P (1998), `Quantitative competitive PCR for the STUDER E, RHYNER C, LU detection of genetically modified soybean and maize', Zeitschrift fuÈ r Lebensmitteluntersuchung und - forschung A, 207, 207±213.

In the identification phase it is very important to know the correct sequences of the transgenic constructs contained in all the varieties of GMO analysed to design specific primers for the amplification of specific DNA sequence characteristics of each GMO. For unequivocal identification, primer selection has to be based on target sequences that are characteristic for the individual transgenic organism. Examples include the cross border regions between integration site and transformed genetic element of a specific GMO, or the cross border regions between different elements constituting the entire transgenic constructs such as promoter and intron or gene and terminator, etc.

NIELSEN P E, EGHOLM M, BERG R H, BUCHARDT O (1991), `Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide', Science, 254, 1497±1500. NUOVO G J (1994), PCR in situ hybridisations: protocols and applications, New York, Raven Press. ) PCR Primer: A Laboratory Manual, Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press, 235±248. éRUM H, NIELSEN P E, EGHOLM M, BERG R H, BUCHARDT O, STANLEY C (1993), `Single base pair mutation analysis by PNA directed PCR clamping', Nucleic Acids Research, 21, 5332±5336.

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