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By Nejat Duzgunes

Liposomes are mobile constructions made from lipid molecules. vital as a mobile version within the learn of simple biology, liposomes also are utilized in scientific functions resembling drug supply and virus stories. *Methods in Liposome Preparation*Physiochemical Characterization of Liposomes

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D. Heath and D. Alford in the laboratory of the late D. 11). 1 mol of inorganic phosphate. 1 mol of inorganic phosphate; Sigma, St. Louis, MO) are also pipetted in triplicate. 4 ml of a 10 N solution) is added to each tube from a polypropylene dispenser jar, and the tubes are  placed for 30 min in a 160–170 heating block with inserts for the test tubes. The tubes are then taken out of the heating block and allowed to cool. 1 ml of a 9% solution) is added to each tube, using a pipettor or repeater pipette (Pipetman or Eppendorf).

Papahadjopoulos, Biochim. Biophys. Acta 557, 9 (1979). 3 M. J. Hope, M. B. Bally, L. D. Mayer, A. S. Janoff, and P. R. Cullis, Chem. Phys. Lipids 40, 89 (1986). 4 F. Szoka, F. Olson, T. Heath, W. Vail, E. Mayhew, and D. Papahadjopoulos, Biochim. Biophys. Acta 601, 559 (1980). 2 METHODS IN ENZYMOLOGY, VOL. 367 Copyright 2003, Elsevier Inc. All rights reserved. 00 4 methods of liposome preparation [1] removal of residual solvent can be tedious. Detergent dialysis techniques are also subject to similar practical difficulties associated with lipid solubility and complete removal of detergent.

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