Download Mammary Stem Cells: Methods and Protocols by Maria del Mar Vivanco PDF

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By Maria del Mar Vivanco

The id of standard and breast melanoma stem cells has provided a brand new imaginative and prescient of this heterogeneous affliction and new hopes for its diagnosis and therapy. This quantity offers an summary of contemporary advancements in mammary stem cellphone learn and discusses the various different types of ways utilized by researchers to enquire the homes and capabilities of mammary stem cells. the start chapters supply readers with an creation to mammary stem cells, and the strategies used to represent stem cells and isolate them through fluorescent activated telephone sorting. the following few chapters talk about DNA and mRNA sequencing, proteomic concepts to aid profile cells, lentiviral cellphone transduction for gene expression, and in vivo lineage tracing. the ultimate few chapters are devoted to following stem cells from their preliminary area of interest to the hot microenvironment at their metastasis web site, and to learning those cells utilizing actual and mathematical techniques. Written within the hugely profitable Methods in Molecular Biology series structure, the chapters comprise the type of particular description and implementation suggestion that's an important for purchasing optimum leads to the laboratory.

Authoritative and state-of-the-art, Mammary Stem Cells: equipment and Protocols goals to assist participants of the medical group discover the habit of stem cells and the way to paintings with them for you to consultant the layout of recent and complimentary suggestions to be utilized within the health facility with the last word finish aim of scuffling with breast cancer.

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Extra info for Mammary Stem Cells: Methods and Protocols

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Besides the frequently utilized NODSCID mice, use of SCID/Beige and NSG mice that lack T-cell, B-cell, and NK cell function for xenotransplantation has been proved to increase the efficiency to establish tumor xenografts of human breast cancers. Recently, Michael Lewis’ team at Baylor College in Houston established a large cohort of 32 stably transplantable xenograft lines in SCID/Beige and NSG mice. These tumor xenograft lines represent different breast cancer subtypes including triple negative (ER−PR−HER2−), HER2-positive (ER−PR−HER2+), ER-positive (ER+PR−HER2−), ER/PR double positive (ER+PR+HER2−), and triple-positive (ER+PR+HER2+), and provide a renewable, quality-controlled tissue resource for preclinical studies to investigate treatment response and metastasis [196].

References 1. Sternlicht MD et al (2006) Hormonal and local control of mammary branching morphogenesis. Differentiation 74(7):365–381 2. Hinck L, Silberstein GB (2005) Key stages in mammary gland development: the mammary end bud as a motile organ. Breast Cancer Res 7(6):245–251 3. Hennighausen L, Robinson GW (2005) Information networks in the mammary gland. Nat Rev Mol Cell Biol 6(9):715–725 4. Smalley M, Ashworth A (2003) Stem cells and breast cancer: a field in transit. Nat Rev Cancer 3(11):832–844 5.

Nat Med 15(8):907–913 Eirew P et al (2008) A method for quantifying normal human mammary epithelial stem 40 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. Ming Luo et al. cells with in vivo regenerative ability. Nat Med 14(12):1384–1389 Keller PJ et al (2011) Defining the cellular precursors to human breast cancer. Proc Natl Acad Sci U S A 109:2772–2777 Visvader JE (2009) Keeping abreast of the mammary epithelial hierarchy and breast tumorigenesis. Genes Dev 23(22): 2563–2577 Bonnet D, Dick JE (1997) Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell.

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