Download Membrane proteins. Volume 557, Engineering, purification and by Arun K. Shukla PDF

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By Arun K. Shukla

Membrane Proteins - Engineering, Purification and Crystallization, a quantity of Methods In Enzymology, encompasses chapters from the best specialists within the zone of membrane protein biology. The chapters supply a quick evaluate of the themes coated and likewise define step by step protocol for the viewers. Illustrations and case instance photographs are incorporated anyplace applicable to assist the readers comprehend the schematics and common experimental outlines.

  • Volume of Methods In Enzymology
  • Contains a suite of a various array of themes within the quarter of membrane protein biology starting from recombinant expression, isolation, useful characterization, biophysical stories and crystallization

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Tapasin and ERp57 form a stable disulfide-linked dimer within the MHC class I peptide-loading complex. EMBO Journal, 24(20), 3613–3623. Pedelacq, J. , Liong, E. , Kim, C. , Rho, B. , et al. (2002). Engineering soluble proteins for structural genomics. Nature Biotechnology, 20(9), 927–932. Prive´, G. G. (2007). Detergents for the stabilization and crystallization of membrane proteins. Methods, 41(4), 388–397. Rizvi, S. , & Raghavan, M. (2010). Mechanisms of function of tapasin, a critical major histocompatibility complex class I assembly factor.

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Grandea, A. , et al. (1997). A critical role for tapasin in the assembly and function of multimeric MHC class I-TAP complexes. Science, 277(5330), 1306–1309. , & Tampe´, R. (2013). Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes. PLoS One, 8(6), e67112. , & Tampe´, R. (2010). ABC proteins in antigen translocation and viral inhibition. Nature Chemical Biology, 6(8), 572–580. Peaper, D. , Wearsch, P. , & Cresswell, P. (2005).

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