Download MicroRNA Expression Detection Methods by Zhiguo Wang, Baofeng Yang PDF

Posted by

By Zhiguo Wang, Baofeng Yang

This e-book presents entire descriptions of the cutting edge ideas and methodologies for detecting miRNA expression, their program in miRNA learn and their power as instruments for scientific analysis and diagnosis.

It starts off with the creation of the general suggestion and methods of miRNA expression detection tools emphasizing the necessity of a wide selection of those easy methods to go well with particular specifications for learn and scientific exam in laboratories. within the following, every one unmarried bankruptcy makes a speciality of an autonomous, exact approach to miRNA detection and is split into 5 subsections: precis, creation, protocol (including fabrics, tool, reagent, and procedure), software and challenge, and reference. the improvement of the method, the guidelines in the back of it and the mechanisms underlying the strategy are given within the advent of every bankruptcy. The step by step protocols are unique within the protocol part. Then, the functions and barriers of the equipment are mentioned. eventually, the literature citations are indexed in reference part. Schematic diagrams are integrated the place wanted and acceptable for higher illustrating the primary of the methodologies. additionally, flowcharts also are supplied to stipulate the protocols for every of the miRNA expression detection methods.

This publication is aimed to focus on either researchers concerned with miRNA study of any fields in universities and examine associations and scientific practitioners who're attracted to constructing or using miRNA profiling as a complementary and another method for medical analysis of human disease.

Show description

Read Online or Download MicroRNA Expression Detection Methods PDF

Similar molecular biology books

Advanced molecular biology: a concise reference

This booklet emphasises the unifying ideas and mechanisms of molecular biology, with widespread use of tables and bins to summarise experimental information and gene and protein capabilities.


This quantity is devoted to an outline of the tools, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the information, intrumentation, and strategies underlying all cryo-EM ways which enable practictioners to simply stream among them. inside every one part, the articles are ordered in response to the commonest symmetry of the pattern to which their tools are utilized.

DNA Structures Part A: Synthesis and Physical Analysis of DNA

To appreciate the constructions and dynamics of DNA and its interactions with ligands, a whole research of its confirmational flexibility is needed. This quantity and its better half, quantity 212 of equipment in Enzymology, collate in a single resource the entire equipment and techniques had to do so aim

Applications of Chimeric Genes and Hybrid Proteins Part A: Gene Expression and Protein Purification

The significantly acclaimed laboratory usual for greater than 40 years, tools in Enzymology is among the such a lot hugely revered guides within the box of biochemistry. due to the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless correct today--truly a vital booklet for researchers in all fields of lifestyles sciences

Extra resources for MicroRNA Expression Detection Methods

Example text

Zanette et al. (2007) described up-regulation of miR-331, miR-29a, miR-195, miR-34a, and miR-29c in CLL. Hodgkin Lymphoma Hodgkin lymphoma (HL) is derived from preapoptotic germinal center B cells, although a general loss of B cell phenotype is noted. HL is one of the most frequently occurring lymphomas, with an annual incidence rate of three to four new cases per 100,000 persons in the Western world. Using quantitative reverse transcription–polymerase chain reaction and miRNA microarray, we determined the miRNA profile of HL and compared this with the profile of a panel of B-cell non–Hodgkin lymphomas.

The dissection of these relationships will be valuable for studying the functions of these miRNAs and their mechanisms in diseases and can be used to discovering novel disease-associated miRNAs. For example, 5 of the 6 papers revealed a down-regulation of let-7 in cancers, which is consistent with previous report, while all papers reported an up-regulation of let-7 in Alzheimer’s disease (see our HMDD database). 1. miRNAs tend to show similar or different dysfunctional evidences for the similar or different disease clusters, respectively.

Altered miR-205 expression has also been reported in various cancers (Feber et al. 2008; Gottardo et al. 2007; Sempere et al. 2007; Iorio et al. 2007; Volinia et al. 2006). ), the adult heart undergoes remodeling process and hypertrophic growth to adapt to altered workloads and to compensate for the impaired cardiac function. Hypertrophic growth manifests enlargement of cardiomyocyte size and enhancement of protein synthesis through the activation of intracellular signaling pathways and transcriptional mediators in cardiac myocytes.

Download PDF sample

Rated 4.38 of 5 – based on 11 votes