Download Nucleic Acid Crystallography: Methods and Protocols by Eric Ennifar PDF

Posted by

By Eric Ennifar

This quantity comprises entire and up to date assurance of all nucleic-acid-specific steps utilized in X-ray crystallography, from macromolecule creation to constitution choice. Chapters devoted to RNA training and crystallogenesis may be of curiosity to newcomers, whereas chapters enthusiastic about facts assortment, phasing and refinement could be really valuable to researchers with a better point of craftsmanship. numerous useful case stories also are awarded within the final a part of the ebook. Written within the hugely winning Methods in Molecular Biology series structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and tips about troubleshooting and averting identified pitfalls.
Authoritative and thorough, Nucleic Acid Crystallography: tools and Protocols provides protocols which are aimed toward either researchers and scholars who're attracted to the structural biology of DNA or RNA, by myself or in advanced with proteins or ligands.

Show description

Read Online or Download Nucleic Acid Crystallography: Methods and Protocols PDF

Similar crystallography books

Thermal Decomposition of Solids and Melts: New Thermochemical Approach to the Mechanism, Kinetics and Methodology (Hot Topics in Thermal Analysis and Calorimetry)

This e-book covers the result of investigations into the mechanisms and kinetics of thermal decompositions of reliable and liquid components at the foundation of thermochemical analyses of the strategies. within the framework of the proposed rules, the most beneficial properties of those reactions are defined and lots of difficulties and weird phenomena, that have gathered during this box are interpreted.

Inorganic and Organometallic Polymers. Macromolecules Containing Silicon, Phosphorus, and Other Inorganic Elements

Content material: An creation to inorganic and organometallic polymers / Kenneth J. Wynne -- Polysilane excessive polymers : an summary / Robert West and Jim Maxka -- Polycarbosilanes : an outline / Dietmar Seyferth -- Soluble polysilane derivatives : chemistry and spectroscopy / R. D. Miller, J. F. Rabolt, R.

Introduction to Structural Chemistry

A concise description of versions and quantitative parameters in structural chemistry and their interrelations, with 280 tables and >3000 references giving the main updated experimental facts on power features of atoms, molecules and crystals (ionisation potentials, electron affinities, bond energies, heats of part transitions, band and lattice energies), optical homes (refractive index, polarisability), spectroscopic features and geometrical parameters (bond distances and angles, coordination numbers) of drugs in gaseous, liquid and stable states, in glasses and melts, for varied thermodynamic stipulations.

Phosphate Fibers

Phosphate Fibers is a unique unique account of the invention, chemistry, synthesis, homes, manufacture, toxicology, and makes use of of calcium and sodium calcium polyphosphate fibers. writer Edward J. Griffith-the inventor and developer of this secure, biodegradable material-takes a multidisciplinary method of this topic, contemplating the social, criminal, clinical, and commercial matters surrounding using asbestos and different mineral fibers.

Extra resources for Nucleic Acid Crystallography: Methods and Protocols

Example text

Serganov A, Patel DJ (2012) Metabolite recognition principles and molecular mechanisms underlying riboswitch function. Annu Rev Biophys 41:343–370 10. Milligan JF, Groebe DR, Witherell GW et al (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res 15:8783–8798 11. Pikovskaya O, Serganov AA, Polonskaia A et al (2009) Preparation and crystallization of riboswitch-ligand complexes. Methods Mol Biol 540:115–128 12. Peselis A, Serganov A (2012) Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitch.

1, step 7. 2. Run the test reactions for 3–4 h at 37 °C. The appearance of a white precipitate due to magnesium pyrophosphate is most of the time visible and can be used to monitor the transcription efficiency. 3. Store at −20 °C or analyze immediately by SDS-PAGE. 0. 46 Clément Dégut et al. 4. To analyze the products of transcription, prepare a SDS-PAGE gel using the same materials as those used for protein SDSPAGE analysis. , a 14 % gel for a 92 nucleotides tRNA. 8, 50 μL SDS 20 % and adjust the volume to 10 mL with water.

6. 15 μL volumes of the RNA and crystallization solutions into the wells of the plate. Seal the plate with Clear Seal film. Store the plate at the desired temperature. 7. Allow the drops in the plates to equilibrate for at least 1 day before checking them with a stereomicroscope at a magnification range of ~5–10×. A polarizing filter is highly recommended for locating small crystals (Fig. 3a, b, g, h). 8. Once conditions that produce crystals have been determined, reproduce crystals in 2 μL hanging drops.

Download PDF sample

Rated 4.76 of 5 – based on 25 votes