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Extra info for Physical properties of chemical compounds. / , A systematic tabular presentation of accurate data on the physical properties of 511 organic cyclic compounds
Other solvent systems offering advantages in some special cases include: benzene-acetone ( 9 : 1 , 1:1), benzene-ether (6:4), benzene-methanol (95:5), benzene-ethyl acetate (8:2, 1:1, 3:7), ether, ether-dimethylformamide (99:1), chloroform-ether (6:4), chloroform-acetone (7:3), ethyl acetate, and ethyl acetatemethanol (99:1). Alumina or Celite with 5 per cent gypsum added may be used as adsorbing material instead of silicic acid. 38 V. SPECIFICITY OF C O L O R REACTIONS In paper-chromatographic analysis, the detection reactions are just as important as the mobilities themselves.
48 R. NEHER TABLE I. MEAN ^-VALUES OF 4 STANDARD STEROIDS IN ZAFFARONI- AND BUSH-TYPE SOLVENT SYSTEMS Zaffaroni-type* Systems, 22° C P/Lig P/Cy-Be (1 : 1) F/Cy P/MCy Carb/MCy F/Cy-Be (1:1) P/Tol F/Be F/Be-CHCla (1:1) F/CHCk F/EBW F/BW F/BBW Bush-type Systems^ % stat. 48 Temp. °C A Bi Progesterone Cortexone Corticosterone Propylenglycol Carbitol Dioxane Ethyl acetate-butyl acetatewater (15: 85:5) F = Formamide BW = Butylacetate-water(100:5)22 BBW == n-Butanol-butyl acetatewater (15: 85: 5) Lig = Ligroin Cy = Cyclohexane Be = Benzene MCy = Methylcyclohexane Toi = Toluene 38 38 38 38 38 38 38 38 38 t Concentration of stationary phase in impregnating solution t A : Petroleum ether-methanol-water (100: 80: 20) B3 : Petroleum ether-benzene-methanol-water (33: 17: 40: 10) Bi : Petroleum ether-toluene-methanol-water (25: 25: 35: 15) BLi : Petroleum ether-benzene-methanol-water (30: 70: 50: 50) E 9 : Hexane-tert.
Finally, in each table the steroids have been arranged according to their molecular formulae, listed in order of progression ; where the molecular formula is the same, they have been listed in alphabetical order by reference to their systematic names. * Since the completion of the manuscript in November 1959 many new values and further compounds have been inserted in or added to the tables, but this was not possible in the case of the figures. 38 CHROMATOGRAPHIC MOBILITIES 39 Excluding the 17 phenolic steroids ("C J8 steroids"), which are compiled separately in Table XXII and which can easily be identified using ferric chloridepotassium ferricyanide2 as a reagent, the 227 neutral steroids listed here can be readily classified using the absorption at 240 m/χ (UV), the reducing power for blue tetrazolium (BT) and the color reaction with m-dinitrobenzene (DNB).