By A. D. Miller (auth.), Nicholas Muzyczka Ph.D. (eds.)
In the prior ten years there was huge, immense growth within the improvement of eukaryotic viral vectors. usually, those vectors were built for one in every of 3 purposes: to accomplish excessive degrees of expression of a selected gene product (poxvirus, baculovirus, and adenovirus), to clone eukaryotic genes together with practical assays (Epstein-Barr virus), of to be used as supply autos for the strong creation of overseas genes into mammalian cells (retroviruses, Epstein-Barr virus, and adeno-associated virus). every one vector has its strengths and weaknesses which are rooted within the occasionally bewildering stra tegies that the mother or father viruses use for propagation. nobody of those vectors is suitable for all the difficulties mole cular biology laboratory is probably going to come across, and few folks are a professional within the molecular virology of all of those viruses. This quantity represents an test by means of the authors to assem ble a evaluation of those vectors in a single position and in a sort valuable to laboratories that don't unavoidably have event with eukaryotic viruses. in actual fact, any virus should be converted to function a vector for a few reasons, and it was once impossible to incorporate an outline of all of those. furthermore, one eukaryotic vector, SV40 (the first one developed), has been reviewed so extensively that we observed no cause to incorporate it here.
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Extra resources for Viral Expression Vectors
Proc NatI Acad Sci USA 83: 8122-8126 Fuerst TR, Earl PL, Moss B (1987) Use of a hybrid vaccinia virus T7 RNA polymerase system for expression of target genes. Mol Cell BioI 7: 2538-2544 Fuerst TR, Fernandez MP, Moss B (1989) Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector. Proc NatI Acad Sci USA 86:2549-2553 Gillard S, Spehner D, Drillien R, Kirn A (1986) Localization and sequence ofa vaccinia virus gene required for multiplication in human cells.
Selection and Screening of Recombinant Virus Plaques ............................ 28 30 31 31 4 Other Poxvirus Vectors .............................. , ......................... 33 5 Hybrid Vaccinia Virus/Bacteriophage T7 Expression Systems. . . . . .. . . . . 33 6 Regulated Gene Expression ..................................................... 34 7 Uses of Recombinant Vaccinia Viruses ........................................... 34 8 Precautions. . . . . . . . . . . . . .
1982; PANICALI and PAOLETTI 1982). Another approach involves the cotransfer of a dominant selectable marker along with the desired foreign gene. This is accomplished by using vaccinia virus DNA to flank both the marker gene and the desired foreign gene. The prokaryotic neomycin-resistance (FRANKE et al. 1985) and guanine phosphoribosyltransferase (gpt) (BOYLE and COUPAR 1988b; FALKNER and Moss 1988) genes, which provide resistance to G418 and mycophenolic acid, respectively, have been employed for this purpose.